Reverse Phase Protein Arrays for Cell Signaling: Using the InnoScan 710-IR Scanner Improves Sensitivity and Dynamic Range

Reverse Phase Protein Arrays (RPPAs) are increasingly used to study cell signaling as they are highly multiplexed and allow precise quantification of protein of interests in biological samples. The InnoScan 710-IR scanner combines infrared detection with high sensitivity and quality images. This application note compares the performance of the InnoScan 710-IR to its main competitor infrared scanner. It is shown that the InnoScan 710-IR improves the signal to noise ratio by a factor of up to 2.7 especially for low concentrations of arrayed cell lysates and extends the dynamic range compared to its competitor.