RNA Extraction from Aspergillus parasiticus mycelium

Recent genomic efforts on toxigenic and nontoxigenic Aspergillus species have advanced our understanding of the biology and genetics of these filamentous fungi. However, it is clear that these complex experiments suffer greatly from the variability in the quality of RNA between each replicate and it is critical to establish a platform to isolate high quality RNA for use in both microarray and qRT-PCR. This application note describes RNA isolation of A. parasiticus during a simple carbohydrate shift.