RNA Quantification Using the GoTaq® RT-qPCR Systems

Promega GoTaq^® products for RNA quantification offer two dye-based systems for accurate, sensitive, broad-range quantification of RNA targets. This application note demonstrates the successful amplification of 11 differentially expressed mRNA targets using the GoTaq^® 1-Step RT-qPCR System. One of these targets was also amplified to demonstrate equivalent performance between the GoTaq^® 1-Step and GoTaq^® 2-Step RT-qPCR Systems. It was found that reactions with the GoTaq^® RT-qPCR Systems have a higher change in fluorescence and earlier Cq values than other RT-qPCR systems.