Robust Routine Amplification of Long DNA Fragments Using KAPA Biosystems LongRange HotStart and HiFi HotStart

This application note describes two solutions for genetic testing of long amplicons from KAPA Biosystems: LongRange HotStart and HiFi HotStart PCR kits. Some genetic and pharmacogenetic tests require the routine amplification of long DNA fragments from complex genomic targets. Assays targeting fragments >3 kb often require careful optimization of reaction parameters, such as Mg2+, primer and template concentrations and cycling parameters. KAPA LongRange HotStart offers robust amplification in single and multiplex assays, whereas KAPA HiFi HotStart is capable of amplifying human genomic targets >10 kb with an error rate up to 50 times lower than long-range enzyme blends.