Routine High-Fidelity Amplification of Longer DNA Fragments is Challenging Due to the Low Processivity and Lack of Robustness of Most Proofreading DNA Polymerases

This application note demonstrates the KAPA HiFi DNA Polymerase, which is a novel, engineered DNA polymerase with the highest fidelity of all commercially available proofreading enzymes. When used with optimal parameters, it enables robust amplification of long and difficult targets from complex templates, high yields and improved sensitivity – in shorter cycling times than required for other proofreading enzymes.