Running Kinase Assays in Kinetic Mode with the Transcreener® ADP2 Assay

This application note describes how to run the Transcreener® ADP2 assay in kinetic, or continuous mode. Using this mode of measurement reduces the number of experiments required to optimize conditions for a new enzyme; e.g., incubation time and temperature, enzyme concentration, etc. Running assays in real time also helps save on the cost of reagents and provides a check on assay linearity.