Sample Preparation for Radio Immunoassay with Vivaspin 2 Ultrafiltration Devices

To obtain antiserum of high quality for antibody production, it is necessary to closely monitor the immunization of animals. A common method for controlling the immunization is performing a radio immunoassay in which the host’s antiserum is diluted and incubated with a radioactive tracer. This application note demonstrates that Vivaspin 2 ultrafiltration devices with a 50 kDa MWCO PES membrane can be used to separate the complex from unbound tracer and measured without further precipitation.