Sarcosine: Separation of Potential Urine Biomarker from Isobaric ß-alanine

Since sarcosine is considered a potential biomarker for prostate cancer risk and aggressiveness, it is essential to resolve and accurately quantify this compound in the presence of isobaric (same m/z) beta-alanine. This application note describes a sensitive, specific, quantitative, and reproducible LC-MS method, using a Cogent Diamond Hydride™ column, for the separation of sarcosine from beta-alanine in serum and urine samples.