SARS-CoV-2 spike protein mutagenesis

Recombinant spike (S) proteins are a critical resource for functional and structural SARSCoV-2 studies. In this application note, DNA Script demonstrates the use of primers produced with DNA Script's novel Enzymatic DNA Synthesis (EDS) technology in a pragmatic, site-directed mutagenesis strategy for the production of optimized and variant SARS-CoV-2 spike protein expression plasmids. EDS primers supported efficient multi-site mutagenesis and Sanger sequencing confirmation of mutations and cloned inserts. Rapid access to custom oligos enabled predictable project schedules amidst a global synthetic DNA supply bottleneck.