Scalable Protein Production Using Flow Electroporation

This technical note reviews the key features of MaxCyte transfection, including cell type flexibility, scalability, consistency, and high-yield protein production that makes this an ideal TGE platform for use in early-phase candidate identification as well as for generating the gram-level antibody quantities needed for late-stage pharmacolgy formulation, stability, and process and purification development. Case studies are presented for gram-scale IgG production in CHO cells, diabody production, and insect cell VLP production. Comparisons with baculovirus expression and lipid-based transfections are shown.