Scouting Protein Purification Conditions Using Vivapure Centrifugal Ion Exchange Membrane Absorbers

For separation and purification of proteins from biological samples, different characteristics of the target protein are exploited. This application note demonstrates the performance of Vivapure IEX Mini spin columns for evaluation of optimal purification conditions of cloned SH2 domains from an E. coli lysate in a two step procedure. This protocol can be employed for finding a purification method based on ion exchange chromatography for a given target protein.