Selecting the Right Tool for In-Gel and In-Solution Protein Digestion

A variety of problems can occur with in-gel and in-solution protein analysis; poor peptide recovery is common in in-gel digestion whereas incomplete digestion is often seen in in-solution digestion. In this application note, a tailored method for in-gel protein digestion is employed using ProteaseMax, a mass spectrometry compatible surfactant, aimed at minimizing missed tryptic cleavages, leading to increased protein identifications, higher accuracy of protein quantitation and better analytical reproducibility.