Separating Fat-Soluble Vitamins by Reversed Phase HPLC, using Sigma-Aldrich Supelco Discovery Columns

Food manufacturers routinely analyze vitamins in their products to determine quantitative levels and to monitor changes that have occurred in food processing. HPLC is preferred for vitamin separation because of its high selectivity. In this application note, various mixtures of fat-soluble vitamins in A, D, and E groups were studied by reversed-phase HPLC using Sigma-Aldrich Supelco Discovery columns.