Separating Water-Soluble Vitamins by Reversed Phase HPLC using a Discovery C18 Column by Sigma Aldrich Supelco

Manufacturers routinely analyze vitamins in their products to determine quantitative levels and to monitor changes that have occurred in processing. HPLC is the preferred technique for vitamin separation because of its high selectivity. In this application note, water-soluble vitamins were separated by reversed-phase HPLC on a Discovery C18 column.