Separation of Intact Monoclonal Antibody Sialylation Isoforms by pH Gradient Ion-Exchange Chromatography

This application note demonstrates high-resolution separation of intact protein sialylation isoforms using the Thermo Scientific™ ProPac™ SCX-10 column on a Thermo Scientific™ Dionex™ UltiMate™ 3000 Dual BioRS LC System with a salt or pH gradient.