Separation of Lipids by Normal Phase Flash Chromatography

Lipid mixtures can be difficult to separate and frequently require multiple procedures to achieve a high purity. This application note demonstrates how this difficult procedure can be performed easily and efficiently with an Isco Normal Phase RediSep® column and CombiFlash® chromatography system. For this study, a mixture was prepared containing non-polar lipids, glycolipids, and phospholipids.