Separation of water-soluble vitamins on the agilent InfinityLab Poroshell 120 Aq-C18 Column

In this application note, Agilent Technologies describes how ten vitamin B compounds plus ascorbic acid and caffeine were analyzed with an Agilent InfinityLab Poroshell 120 Aq-C18 column using a phosphate buffer and acetonitrile gradient — resulting in good resolution and peak shape.