Separations of Peptide Antibiotics by Reversed Phase HPLC, using Sigma-Aldrich Supelco Discovery Columns

Antibiotic resistance has become a major clinical problem so there is an urgent need to develop agents that act via novel mechanisms. In this application note, Bacitracin A and Virginiamycin M1 were analyzed by HPLC, using Discovery C18, Discovery C8, and Discovery RP-AmideC16 columns. These columns offer excellent resolution for this application.