Separations of Tetracycline Antibiotics by Reversed Phase HPLC, using Sigma-Aldrich Supelco Discovery Columns

There are more than 100 antibiotics on the market today, and many more are in development. Resistance to antibiotics is a significant problem; an antibiotic that takes a decade to bring to market can induce resistance within months of its introduction into clinical practice. Therefore, it is critical to monitor the level of antibiotics given to humans and animals and HPLC is a powerful tool for isolation and quantification of such. In this application, five tetracycline antibiotics were analyzed by HPLC, using Discovery C18, Discovery C8, and Discovery RP-AmideC16 columns.