Shortening the Timeline for Drug Development by Enabling Large-Scale TGE in CHO Cells and High Producing Stable CHO Cell Line

This application note demonstrates the use of MaxCyte electroporation for creation of stable CHO pools and the rapid generation of high-yield stable cell lines and the TGE ability of MaxCyte flow electroporation to produce antibody titers up to 2.7 g/L. To reduce early-stage development costs and lower late-stage attrition rates, many biopharmaceutical companies have turned to transient gene expression (TGE) rather than developing stable cell lines for early-stage development work. With the ability to produce gram-level quantities of antibodies, Maxcyte’s flow electroporation extends the applicability of TGE to later stages of antibody development.