Simple and Fast Quantitation of Nicotinic Acid and Nicotinamide in Human Plasma by Applying Impact™ Protein Precipitation Plate Technology with Gemini® 3 μm C18 HPLC Columns

In this application note, nicotinic acid and nicotinamide were extracted from human plasma by performing a rapid protein precipitation using Impact Protein Precipitation Plates followed by HPLC analysis using a Gemini 3 μm C18 100 x 4.6 mm HPLC column and positive polarity ESI LC/MS/MS system. Impact technology offers easy, fast protein removal while providing maximized recovery of the target analytes. The Gemini 3 μm C18 HPLC column produced excellent chromatographic resolution, sensitivity, and high peak capacities.