Simple Conversion from ELISA to Luminex® Using the AMG™ Activation Kit from Anteo Technologies.

Luminex® MagPlex® Microspheres may be coupled with antibodies specified for use in ELISA using Anteo Technologies’ AMG™ Activation Kit. This kit uses Mix&Go™ technology to strongly couple antibodies to multiplex microspheres such as Luminex® MagPlex® Microspheres. This application note gives the example of coupling two anti-cytokine antibodies to MagPlex Microspheres, followed by a multiplex immunoassay using the Luminex® system with the matched detection antibodies and standards.