Simple Enrichment of Phosphopeptides using TiO2 Magnetic Neads

Phosphorylation is a common, reversible post-translational modification involved in the regulation of many essential biological processes such as cell signaling, which is of prime importance for the study of various disease states such as cancer. Knowledge about phosphorylation may help to understand disease progression. Phosphoproteins and phosphopeptides are usually present at very low concentrations and ionize poorly, making their detection by mass spectrometry (MS) difficult. In this application note read how porous agarose TiO2 beads with paramagnetic properties can be used as a tool to enrich phosphopeptides.