Simple large volume injection method for trace-level glyphosate in bottled drinking water

The analysis of glyphosate in water is challenging primarily because it is not retained on reversed-phase columns and its response is often poor due to chelation with metal surfaces along the sample path through the LC-MS/MS system. Derivatization and ion-pairing reagents are often used to improve performance, but they can be unfriendly to the instrument and require additional sample preparation time. Using a Raptor Polar X column is a better alternative because the novel stationary phase has an affinity for polar anionic compounds, such as glyphosate, and it provides both effective retention and efficient elution.