Simplifying High Throughput 3D Spheroid Growth and Shrinkage Assays Using Live Content Imaging

Using fluorescently labeled cells and IncuCyte ZOOM™, this poster shows the assembly and validation of fully-kinetic, spheroid growth and shrinkage assays. IncuCyte ZOOM™ metrics (spheroid size, (area/confluence) and fluorescence intensity (Mean Image Fluorescence, MIF)) are informative, and can be readily gathered to monitor spheroid growth and shrinkage over time. These assays should prove useful for medium throughput, quantitative pharmacology of test samples for effects on the growth and shrinkage of tumor cells in 3D.