Simultaneous Analysis of Amino Acids, Acylcarnitines, and Succinylacetone in Dried Blood Spots for Research Using Nonderivatized and Derivatized Methods

Flow injection tandem mass spectrometry has been frequently used for analysis of amino acids (AA) and acylcarnitines (AC) in dried blood spots, for inborn errors of metabolism research. Traditional methods can be laborious because they require additional extraction. This application note describes a single extraction step that was used to simultaneously analyze AA, AC, and SUAC in dried blood spots, on the Thermo Scientific™ TSQ Endura™ triple quadrupole mass spectrometer.