Simultaneous Extraction of Ethyl Glucuronide and Ethyl Sulfate from Urine with EVOLUTE AX Prior to LC-MS-MS Analysis

This application note describes the simultaneous extraction of the alcohol metabolites ethyl glucuronide (EtG) and ethyl sulfate (EtS) from human urine using Biotage EVOLUTE AX columns in a range of column sizes followed by LC-MS-MS analysis. This application note demonstrates the simultaneous extraction of both EtG and EtS in the same assay and represents further optimization of AN718 which showed single EtG extraction without EtS.