Simultaneous Quantitative Determination of Ethyl Glucuronide and Ethyl Sulphate in Human Urine using UPLC®/MS/MS

The aim of this note is to develop and validate a simple and rapid UPLC/MS/MS method for the simultaneous quantitative determination of ethyl glucuronide (EtG) and ethyl sulphate (EtS) in human urine. The developed methodology is shown to provide rapid results in a single 4 minute chromatographic run.