Single Cell Sorting of CRISPR/Cas9-Expressing Cells Using the Sony SH800S

By introducing the nuclease Cas9 into cells, along with guide RNA (gRNA) oligomers, it is possible to achieve highly specific genome editing. This application note details how the sort deposition system of the SH800S facilitates high-throughput sorting of successfully transfected Cas9/gRNA cells, into 96- and 384- well devices.