Single Nucleotide Polymorphism Detection with the iPLEX® Assay and the MassARRAY® System

The MassARRAY® System from Agena Bioscience™ is a scalable platform with a suite of research applications for quantitative and qualitative nucleic acid analysis, enabling flexible assay design and fast time-to-results. This application note describes the iPLEX SNP genotyping and somatic mutation workflow and outlines advances in the protocol that enable: flexible, scalable, and sensitive genotyping in 24-, 96-, or 384-well formats; cost-effective and accurate multiplexing of up to 40 different SNPs in a single reaction well; optimized thermal cycling methods and universal cycling parameters that reduce workflow time to just one day.