Single-stranded DNA in the Qubit™ dsDNA Assays for the Qubit® 2.0 Fluorometer

This study shows that both Qubit™ dsDNA assays detected only 2–10% of pure ssDNA for the majority of the range. Similarly, when an equal mass of ssDNA was added to dsDNA, the results generally changed by less than 10% from the results obtained with dsDNA alone.