Site-Directed Mutagenesis

The KAPAHiFi™ site-directed mutagenesis application note provides a simple and highly efficient mutagenesis method, for use with KAPAHiFi™ HotStart and KAPAHiFi™ DNA Polymerases. This protocol allows a variety of modifications to be made in a gene sequence, using any cloning vector containing an antibiotic resistance gene. In addition, the use of KAPAHiFi™ Hotstart or KAPAHiFi™ DNA Polymerases ensures the high fidelity replication of the plasmid, thereby minimizing polymerase-induced errors.