Size-exclusion chromatography analysis of adeno-associated virus (AAV) preparations using a 450 Å Diol-Bonded BEH Column and fluorescence detection

This application note presents optimized size-exclusion chromatography (SEC) methods that can separate soluble AAV self-associated forms and fragments under non-denaturing conditions for several CMV-GFP control AAV serotypes including AAV1, AAV2, AAV5, AAV6, AAV8, and AAV9.