Sorting of MSCs with CD271 and MSCA-1 Antibodies in a Closed-Cartridge System

Mesenchymal stem cells (MSCs) have raised great expectations in a number of clinical trials studying the regeneration of bone, cartilage, and heart or autoimmune diseases including GvHD, Crohn’s disease, and diabetes. MSCs are an extremely rare cell type in cord blood, adipose tissue, and bone marrow (BM, 0.01%). Several groups worked on the identification of MSCs, using markers such as CD271, Anti-MSCA-1 (TNAP), CD73, and STRO-1.