Stability of Sub-Unit Vaccines Measured with the UNit Label-Free Stability Platform

Adjuvant absorption has been shown to result in some changes to the melting temperature of antigens used in subunit vaccines. This application note describes how the UNit platform uses intrinsic protein fluorescence to determine changes in the conformational stability of proteins which are both in a ‘free’ solution and when adsorbed to an adjuvant. The measurements produced are high throughput in nature and require only a small sample size, and is less time consuming due to the disposable consumables sample holder reducing the need for a cleaning step.