Steroid profiling from dried blood spot samples

Measurement of increased 17-OHP concentration in dried blood spot (DBS) by immunoassays (IA) is associated to analytical interference arising from cross-reactivity of the reagent antibodies with other structurally-related steroid metabolites, particularly 17-hydroxypregnenolone. To allow more specific marker measurement, PerkinElmer reports here an ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for steroid profiling from dried blood spot (DBS) samples. It allows the simultaneous specific determination of 17-OHP, ADIONE, CORT2, and additional steroids such as 21-deoxycortisol (21-DECOL) and 11-DECOL to enable distinction between 21-H and 11β-H deficiency.