Stimulation of human peripheral blood mononuclear cells

Human peripheral blood mononuclear cells (PBMCs) are routinely isolated from blood samples and then used in several fields of research including autoimmune disorders, infectious diseases, vaccine development and cancers. In this application note, learn more about the ELISpot Assay, which monitors ex vivo cellular immune responses to antigenic stimuli. The Agilent BioTek Cytation 7 cell imaging multimode reader is used in conjunction with the Agilent BioTek Gen5 microplate reader and imager software to quantitate changes in cytokine secretion in PBMCs using the colorimetric ELISpot assay format.