Stopped Flow Time‐Resolved Fluorescence Study of Serum Albumin – Curcuminoid Binding

Rapid mixing accessories to perform stopped flow measurements have found application in characterizing interactions and reactions occurring in solution. The recent introduction of very low deadtime electronics coupled with high repetition rate excitation sources have now allowed efficient and fast measurement of fluorescence decays. This application note monitors the interaction of a fluorescent curcuminoid mixture with serum albumin using a stopped flow accessory on a DeltaFlex system with DeltaDiode excitation at 100MHz.