Strategy to Develop Purification Protocols Using ÄKTA™ System

This application note describes a strategy to develop a purification protocol using a Design of Experiment (DoE) approach. DoE is a method to design a study to determine how parameters interact and influence the performance. This method is used to collect more information with higher accuracy in a shorter period of time. The UNICORN™ control system for ÄKTA avant includes DoE functionality that was used to develop the purification protocol.