Streamlining Antibody Development Using Large Scale, CHO Transient Gene Expression (TGE) Followed by Rapid Production of CHO Stable Pools

Antibody production for early stage antibody development activities is commonly conducted using transiently transfected HEK cells to produce adequate quantities of antibody for characterization, while later stage screening and biomanufacturing rely on CHO-based stable cell lines. MaxCyte’s proprietary electroporation technology can greatly shorten the time line of antibody development by enabling large scale transient gene expression (TGE) directly within CHO cells. This poster presents data showing rapid gram-scale antibody production via transient transfection using the MaxCyte STX®. CHOS cells can be transfected with >95% transfection efficiency and cell viability.