Successful Transfer of Size-Exclusion Separations between HPLC and UPLC

Waters’ recent development of HPLC compatible 200Å and 450Å, 3.5 µm particles based on the same diol-coated BEH-particle chemistry as that used in ACQUITY UPLC Protein BEH SEC Columns provides the capability to readily transfer between SE-UPLC and SE-HPLC for the first time. This application note details and provides examples of the methodology that can successfully employed for this method transfer.