TARGATT large knock-in technology

While CRISPR has transformed gene editing, it presents limitations for applications requiring large DNA insertions in mammalian cells, including low knock-in efficiency for fragments over 3–5 kb and risks associated with double-strand breaks.

Explore how TARGATT™ gene knock-in technology from Applied StemCell addresses these challenges through a precise, efficient, and scalable approach. Utilizing a three-component system, TARGATT integrase, a defined H11 safe harbor site, and a donor plasmid enables targeted, single-copy insertion of DNA fragments up to 20 kb. Its unidirectional, site-specific integration ensures stable expression and genomic integrity, making TARGATT a robust solution for complex gene engineering and therapeutic development.