Targeted Exome and Gene Panel Analysis from Low Input and FFPE DNA using Hybridization Capture for Cancer Genome Studies

Hybridization-based target enrichment techniques coupled with Next-Generation Sequencing (NGS) provide a useful and cost-efficient means to study disease specific target regions including whole exomes and gene panels. Clinical samples may be limited in input and of compromised quality due to formalin fixation, however most current NGS library preparation methods require 50-100 ng high quality DNA for such studies. This application note presents an efficient method, which enables high quality target enrichment and variant calling from inputs as low as 1-25 ng.