TDA of the Self-Association Behaviour of Insulin with Concentration using Malvern’s Viscosizer TD

Taylor Dispersion Analysis (TDA) is an orthogonal technique for sizing and stability studies of biomolecules in solution. Whilst compared to the well-established and complementary technique of Dynamic Light Scattering (DLS), the two techniques respond differently to the presence of mixtures. As an important biotherapeutic used in the treatment of Diabetes Mellitus, Insulin provides an interesting case study with which to review the use of TDA as a technique for characterizing the hydrodynamic radius (Rh) and stability of protein-based biopharmaceuticals in solution, and to highlight the differences in data obtained for oligomeric mixtures from DLS measurements.