Ten-Parameter Immunophenotyping of Human Lysed Whole Blood with the Attune NxT Flow Cytometer Allows Identification of Multiple T-Cell Subsets and Myeloid Cells

Immunophenotyping is the analysis of heterogeneous populations of cells within human blood for the purpose of identifying the presence and proportions of various cell populations. Multiparameter flow cytometry is a widely-used method for identifying cell subsets within complex cellular systems. This application note describes the use of the Attune NxT Flow Cytometer for 4-laser and 10-color immunophenotyping analysis of stained human whole blood using a stain-and-lyse protocol.