The Analysis of Vitamin D Metabolites in Serum/Plasma by LC‐MS/MS

Vitamin D exists in two forms, vitamin D2 and vitamin D3. Each undergoes metabolism to form 25‐hydroxyvitamin D2 and 25‐hydroxyvitamin D3. For accurate determination of vitamin D levels in the blood, it is important to distinguish between these metabolites and to separate them from major matrix interferences. In this method, the Raptor^TM ARC‐18 column combines the speed of superficially porous particles (SPP) with the resolution of highly selective USLC® technology to produce a simple and rugged method for the determination of vitamin D metabolites in serum and plasma.