The Determination of CBD and General Cannabinoid Content in Hemp Oils Using HPLC with UV Detection

Medical marijuana generally possesses high levels of the psychotropic tetrahydrocannabinol, d9-THC and lower levels of the non- psychotropic cannabidiol, CBD. Pain mitigation and reduced severity of nausea and seizures are just a few of the therapeutic benefits reported by medical cannabis patients. Little has been done to better understand the chemistry of benefits from CBD. To complicate matters, there is evidence that a combination of CBD, a host of other minor cannabinoids and a complex array of terpenoids may be the most beneficial – called the “entourage effect.” CBD-rich oil has become increasingly popular and is administered via sublingual drops, gel capsules or as a topical ointment.