The effect of column hardware on the analysis of synthetic oligonucleotides by LC-MS

Therapeutic oligonucleotides represent a recent breakthrough in the pharmaceutical industry. Improved reliability to synthetic routes coupled with new drug delivery technologies has given a heightened level of interest in these clinically interesting targets. In this application note from Phenomenex, learn how by minimizing non-specific interactions, the use of BioTi™ hardware not only serves to improve chromatographic performance and reproducibility of oligonucleotide separations, but also maximizes analyte recovery and sensitivity enabling both consistent quantitation and characterization.