The Impact of Low Level Organics on HPLC and LC-MS Baselines of Ultrapure Water

High-purity water comprises by far the largest mobile phase component for most reversed phase HPLC and LC-hyphenated methods. In this study, reversed phase LC/MS analyses were performed on water at five different steps of a water purification chain that was chosen specifically because of its high potential for removing organic contaminants, thus for achieving the lowest HPLC or LCMS baselines.