The Impact of Transfection Mediated Toxicity - Gene Expression and Cytotoxicity Analysis of Transfection Reagents

While plasmid DNA delivery is a widely used method to study cellular functions of proteins of interest, studies to identify nontoxic gene delivery reagents are limited. This application note presents a study which explores the conventional toxicological endpoints, and identifies the minimal-transcriptomic effects, of TransIT®‐LT1, TransIT®‐2020 and Lipofectamine® 2000 Transfection Reagents using quantitative reverse transcriptase PCR (RT‐qPCR) array and pathway analysis software.